Journal: Molecular cancer therapeutics

Article Title: Differential Sensitivity to xCT-inhibition by ME1 expression

doi: 10.1158/1535-7163.MCT-25-0299

Figure Lengend Snippet: a. Tumor growth curves of xenografted KP2 tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=9), LD (30mg/kg) MD (45mg/kg) S2E (n=9), and HD ACXT-3102 (60mg/kg) (n=9) groups. Treated for a total of 10 days. b. Tumor growth curves of xenografted ASPC1 tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=8), LD (30mg/kg) (n=8), MD S2E (40mg/kg) (n=8), and HD ACXT-3102 (60mg/kg) groups. Treated for a total of 10 days. c. Tumor growth curves of xenografted PAUT tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=8), LD (30mg/kg) S2E (n=8), MD ACXT-3102 (45mg/kg) (n=6) groups. Treated for a total of 10 days. d. Tumor growth curves of xenografted SHP77 tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=5), LD (30mg/kg) ACXT-3102 (n=5), MD (45mg/kg), and HD ACXT-3102 (60mg/kg) (n=5) groups. Treated for a total of 10 days. e. Tumor growth curves of xenografted HCT116 tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=8), LD (30mg/kg) (n=8), MD (45mg/kg), and HD ACXT-3102 (60mg/kg) (n=8) groups. Treated for a total of 10 days. f. Tumor growth curves of xenografted RKO tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=14), LD (30mg/kg) S2E (n=14), MD (40mg/kg), and HD ACXT-3102 (60mg/kg) (n=10) groups. Treated for a total of 10 days. g. Tumor endpoint weight for orthoptic KP2 tumors in control (isotonic saline – 0.9% NaCl vehicle), (n=11), and HD ACXT-3102 (60mg/kg) (n=11) groups. Treated for a total of 14 days. h. Tumor endpoint volumes for orthotopic KP2 tumors in control (isotonic saline – 0.9% NaCl vehicle) (n=12), and HD ACXT-3102 (60mg/kg) (n=11) groups. Treated for a total of 14 days. All data are expressed as mean± SEM. Non-significant data noted as ns, p > 0.05, * p<0.05, **p<0.01, ***<0.001. ANOVA testing was performed for all tumor growth analyses.

Article Snippet: Cancer cell lines ASPC1 (RRID: CVCL_0152), HCT-116 (CVCL_0291), RKO (CVCL_0504), SKUT1 (RRID: CVCL_0533), SK-LMS-1 (RRID: CVCL_0628), SHP-77(RRID: CVCL_1693), and NCI-H69 (RRID: CVCL_1579) were obtained in 2018 from the American Type Culture Collection (ATCC, Manassas, VA, Cat. #: CRL-1682, CCL-247, CRL-2577, HTB-115, HTB-88, CRL-2195, and HTB-119).

Techniques: Control, Saline

Journal: Molecular cancer therapeutics

Article Title: Differential Sensitivity to xCT-inhibition by ME1 expression

doi: 10.1158/1535-7163.MCT-25-0299

Figure Lengend Snippet: a. Protein expression of ME1 in colon cancer (RKO, HCT-116), sarcoma (SK-LMS-1, SKUT), and lung cancer (SHP-77, NCI-H69). Band density differences are plotted as ME1 area under the curve normalized to total protein in the capillary (n=3). b. Protein expression of ME1 in ME1 non-expressing wild-type cells (RKO) and overexpressing cells (RKO ME1 OE) and ME1 expressing wild-type cells (ASPC1, HCT-116), and shRNA knockdown cells (ASPC1 ME1 KD, HCT-116 ME1 KD). Band density differences are plotted as ME1 area under the curve normalized to total protein in the capillary (n=3). c. Percent cell viability at 4 hours in ASPC1 and ASPC1 ME1 KD treated with increasing doses of ACXT-3102, measured using YOYO-1 Iodide counts/well, normalized to total cell count (n=3). d. Percent cell viability at 4 hours in HCT-116 and HCT-116 ME1 KD treated with increasing doses of ACXT-3102, measured using YOYO-1 Iodide counts/well, normalized to total cell count (n=3). e. Percent cell viability at 4 hours in RKO wild type and RKO ME1 OE cells treated with increasing doses of ACXT-3102, measured using YOYO-1 Iodide counts/well, normalized to total cell count (n=3). All data are expressed as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. Two-tailed unpaired t-testing was used for analysis in figures a and b and two-way ANOVA testing was used for figures c, d, and e.

Article Snippet: Cancer cell lines ASPC1 (RRID: CVCL_0152), HCT-116 (CVCL_0291), RKO (CVCL_0504), SKUT1 (RRID: CVCL_0533), SK-LMS-1 (RRID: CVCL_0628), SHP-77(RRID: CVCL_1693), and NCI-H69 (RRID: CVCL_1579) were obtained in 2018 from the American Type Culture Collection (ATCC, Manassas, VA, Cat. #: CRL-1682, CCL-247, CRL-2577, HTB-115, HTB-88, CRL-2195, and HTB-119).

Techniques: Sensitivity, In Vitro, Inhibition, Expressing, shRNA, Knockdown, Cell Characterization, Two Tailed Test, Analysis

Journal: Molecular cancer therapeutics

Article Title: Differential Sensitivity to xCT-inhibition by ME1 expression

doi: 10.1158/1535-7163.MCT-25-0299

Figure Lengend Snippet: a. Tumor growth curves of xenografted ASPC1 WT control (isotonic saline – 0.9% NaCl vehicle) (n=10) and 60 mg/kg ACXT-3102 (n=10) groups. Tumor volume generated every 2 days for 18 days. b. Tumor growth curves of xenografted ASPC1 ME1 KO control (isotonic saline – 0.9% NaCl vehicle) (n=10) and 60mg/kg ACXT-3102 (n=10) groups. Tumor volume generated every 2 days for 18 days. c. Tumor growth curves of xenografted RKO WT control (isotonic saline – 0.9% NaCl vehicle) (n=15) and 30 mg/kg ACXT-3102 (n=14) groups. Tumor volume generated every 3 days for 30 days. d. Tumor growth curves of xenografted RKO ME1 OE control (isotonic saline – 0.9% NaCl vehicle) (n=12) and 30 mg/kg ACXT-3102 (n=15) groups. Tumor volume generated every 3 days for 30 days. e. Tumor growth curves of xenografted RKO ME1 OE control (isotonic saline – 0.9% NaCl vehicle) (n=12) and 60 mg/kg ACXT-3102 (n=15) groups. Tumor volume generated every 3 days for 30 days. f. Protein expression of ME1 in RKO and RKO ME1 OE tumors (n=5) after xenografting. Band density differences are plotted as ME1 area under the curve normalized to total protein in the capillary. All data are represented as mean ± SD. *** < 0.001. g. Kaplan Meier overall survival curves of RKO WT xenografted mice demonstrating median survival in control (isotonic saline – 0.9% NaCl vehicle) (21 days) and 30mg/kg ACXT-3102 (59 days). Log-rank test p <0.001. h. Kaplan Meier survival curves of RKO ME1 OE xenografted mice demonstrating median survival in control (isotonic saline – 0.9% NaCl vehicle) (17 days), 30mg/kg ACXT-3102 (17 days), and 60mg/kg ACXT-3102 (35 days). Log-rank test p <0.001. Tumor growth curves are expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA testing was used for figures a-e, two-tailed unpaired t-testing was used for analysis in figure f, and Kalpan Meier survival curves were generated in figures g and h with log rank testing for significance.

Article Snippet: Cancer cell lines ASPC1 (RRID: CVCL_0152), HCT-116 (CVCL_0291), RKO (CVCL_0504), SKUT1 (RRID: CVCL_0533), SK-LMS-1 (RRID: CVCL_0628), SHP-77(RRID: CVCL_1693), and NCI-H69 (RRID: CVCL_1579) were obtained in 2018 from the American Type Culture Collection (ATCC, Manassas, VA, Cat. #: CRL-1682, CCL-247, CRL-2577, HTB-115, HTB-88, CRL-2195, and HTB-119).

Techniques: Over Expression, Inhibition, Sensitivity, In Vivo, Control, Saline, Generated, Expressing, Two Tailed Test, Analysis